GDNF (Animal Free)
GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
Product Specifications
Synonyms
GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
NCBI Gene ID
2668
UniProt
P39905
Accession Number
NP_000505.1
Accession Number mRNA
NM_000514
Chromosomal Location
5p13.1-p12
Reactivity
Human
Cross Reactivity
Human, Monkey, Mouse
Sequence
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Endotoxin
< 0.01 ng/µg of protein (< 0.1 EU/µg)
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Bioactivity
Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.
Length
135
Form
Lyophilized
Molecular Weight
15 kDa
Host or Source
E. coli
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