GDF-5 (BMP-14/CDMP-1, Animal Free)
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant Human GDF-5 (BMP-14/CDMP-1) is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains.
Product Specifications
Synonyms
Growth/Differentiation Factor-5, Bone Morphogenetic Protein-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1) ; GDF5; OS5; LAP4; BMP14; SYNS3
NCBI Gene ID
8200
UniProt
P43026
Accession Number
NP_000548
Accession Number mRNA
NM_000557
Chromosomal Location
20q11.2
Reactivity
Human
Cross Reactivity
Human
Sequence
APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Endotoxin
< 0.01 ng/µg of protein (< 0.1 EU/µg)
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Bioactivity
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 µg /ml.
Length
120
Form
Lyophilized
Molecular Weight
27 kDa
Host or Source
E.coli
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