G-CSF (Animal Free)

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

Product Specifications

Synonyms

CSF3; GCSF; CSF3OS; C17orf33

NCBI Gene ID

1440

UniProt

P09919

Accession Number

NP_000750.1

Accession Number mRNA

NM_000759

Chromosomal Location

17q11.2-q12

Reactivity

Human

Cross Reactivity

Human, Mouse, Plant

Sequence

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Endotoxin

< 0.01 ng/µg of protein (< 0.1 EU/µg)

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Bioactivity

Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.