FGF-acidic (Animal Free)

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant Human FGF-acidic is a 16.8 kDa protein consisting of 141 amino acid residues.

Product Specifications

Synonyms

FGF2; BFGF; FGFB; HBGF-2

NCBI Gene ID

2246

UniProt

P05230

Accession Number

NP_000791.1

Accession Number mRNA

NM_000800.4

Chromosomal Location

5q31

Reactivity

Human

Cross Reactivity

Cow, Human, Mouse, Pig, Rat, Shark, Squid

Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Endotoxin

< 0.01 ng/µg of protein (< 0.1 EU/µg)

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Bioactivity

Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.5 ng/ml in the presence of 10 µg/ml heparin, corresponding to a specific activity of ≥ 2 x 106 units/mg.