FGF-8B

FGF-8 (FGF-8b) is a heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF-8; FGF-8A, FGF-8B, FGF-8E and FGF-8F. The human and murine FGF-8A and B are identical unlike human and mouse FGF8 E and F are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors. Recombinant human FGF-8 (FGF-8b) is a 22.4 kDa protein consisting of 193 amino acid residues.

Product Specifications

Synonyms

FGF8; AIGF; KAL6; FGF-8; HBGF-8

NCBI Gene ID

2253

UniProt

P55075

Accession Number

NP_149355

Accession Number mRNA

NM_033165

Chromosomal Location

10q24

Reactivity

Human/Murine

Cross Reactivity

Chicken, Mouse, Rabbit, Rat, Hamster, Human

Sequence

MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 95% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF- receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.