FGF8a / FGF-8a Protein

In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.

Product Specifications

Synonyms

AIGF Protein; FGF-8 Protein; FGF8 Protein; HBGF-8 Protein; HH6 Protein; KAL6 Protein

Accession Number

NP_149355.1

Reactivity

Human

Cross Reactivity

Human

Assay Protocol

See Datasheet send with the product

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 μg/mL.

Length

183

Form

Lyophilized or frozen liquid

Buffer

20 mM Tris, 500 mM NaCl, pH 8.5.

Reconstitution

See Datasheet

Molecular Weight

21.3 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.