FGF-20

FGF-20 is secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-20 signals through the FGFR 2c and 3c and is expressed during limb and brain development. Recombinant human FGF-20 is a 23.2 kDa protein containing 209 amino acid residues.

Product Specifications

Synonyms

FGF20; FGF-20

NCBI Gene ID

26281

UniProt

Q9NP95

Accession Number

NP_062825.1

Accession Number mRNA

NM_019851

Chromosomal Location

8p22

Reactivity

Human

Cross Reactivity

Monkey, Mouse

Sequence

PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE LYKDLLMYT

Assay Protocol

Reconstitute in 10mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 90% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.