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EGF (Animal Free)

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor) . Recombinant Murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

Product Specifications

Synonyms

Epidermal growth factor; Egf; AI790464

NCBI Gene ID

13645

UniProt

P01132

Accession Number

NP_036974.1

Accession Number mRNA

NM_010113.3

Chromosomal Location

3 G3; 3 65.2 cM

Reactivity

Mouse

Cross Reactivity

Human, Mouse

Sequence

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Endotoxin

< 0.01 ng/µg of protein (< 0.1 EU/µg)

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Bioactivity

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Length

53

Form

Lyophilized

Molecular Weight

6.0 kDa

Host or Source

E. coli

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