EGF

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor) . Recombinant rat EGF is a 6.2 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.

Product Specifications

Synonyms

Epidermal growth factor, Urogastrone, URG

NCBI Gene ID

25313

UniProt

P07522

Accession Number

NP_036974.1

Accession Number mRNA

NM_012842.1

Chromosomal Location

2q42-q43

Reactivity

Rat

Cross Reactivity

Mouse, Rat

Sequence

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.