What is the catalog number for DKK-1 Protein (hFc Tag)?

The catalog number for DKK-1 Protein (hFc Tag) is 600-096-L100.

What sizes are available for DKK-1 Protein (hFc Tag)?

DKK-1 Protein (hFc Tag) is available in the following size(s): 100 µg.

Is DKK-1 Protein (hFc Tag) in stock?

DKK-1 Protein (hFc Tag) is currently made to order / not held in immediate stock. Contact us for lead time.

Are there any special shipping requirements for DKK-1 Protein (hFc Tag)?

DKK-1 Protein (hFc Tag) is requires dry ice shipping.

DKK-1 Protein (hFc Tag)

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Specifications

Synonyms

DKK-1 Protein; SK Protein; UNQ492/PRO1008 Protein

Accession Number

NP_036374.1

Reactivity

Human

Cross Reactivity

Human

Label

HFc-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07μg/mL in the presence of 10 ng/mL of mouse Wnt3a. 2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated on SA Biosensor, can bind Recombinant Human DKK-1 Protein, hFc Tag with an affinity constant of 54.3 nM as determined in BLI assay (Sartorius Octet RED384) .

Length

473

Form

Lyophilized

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

52.5 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.