CD80 / B7-1 / CD28LG Protein (His Tag)

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Product Specifications

Synonyms

B7 Protein; B71 Protein; Cd28l Protein; Cd80 Protein; Ly-53 Protein; Ly53 Protein; MIC17 Protein; TSA1 Protein

Accession Number

Q00609-1

Reactivity

Mouse

Cross Reactivity

Mouse

Label

His-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to bind recombinant mouse CD28 in a functional ELISA. 2.Loaded Recombinant Mouse CD28 Protein on proA Biosensor, can bind Recombinant Mouse B7-1/CD80 Protein with an affinity constant of 1.410 μM as determined in BLI assay (Sartorius Octet RED384) .

Length

219

Form

Lyophilized

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

50 kDa (glycosylated)

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.