CD28 Protein (ECD, mFc Tag)

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Product Specifications

Synonyms

Cd28 Protein

Accession Number

P10747-1

Reactivity

Human /Cynomolgus/Rhesus

Cross Reactivity

Human, Cynomolgus, Rhesus

Label

MFc-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS

Bioactivity

Loaded Recombinant Human, Cynomolgus, Rhesus CD28 Protein on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein with an affinity constant of 24.200 nM as determined in BLI assay (Sartorius Octet RED384) .

Length

119

Form

Lyophilized

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

58.66 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.