CD27 / TNFRSF7 Protein (His Tag)

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Product Specifications

Synonyms

Cd27 Protein; S152 Protein; Tnfrsf7 Protein; Tp55 Protein

Accession Number

NP_001028298.1

Reactivity

Mouse

Cross Reactivity

Mouse

Label

His-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 96 % as determined by SDS-PAGE

Bioactivity

Immobilized CD70 Protein, Rat, at 2 μg/mL (100 μL/well) can bind CD27 Protein, Mouse, the EC50 is 50-280 ng/mL.

Length

173

Form

Lyophilized or frozen liquid

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

35 kDa (glycosylated)

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.