Carboxypeptidase B1 / CPB1 Protein (His Tag)

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Product Specifications

Synonyms

CPB Protein; PASP Protein; PCPB Protein

Accession Number

NP_001862.2

Reactivity

Mouse

Cross Reactivity

Human

Label

His-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to cleave a peptide substrate, Hippuryl-Arg. The specific activity is >1000 pmoles/min/μg.

Length

413

Form

Lyophilized

Buffer

25 mM MES, 100 mM NaCl; pH 6.5

Reconstitution

See Datasheet send with the product

Molecular Weight

47 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.