Artemin
Artemin is a disulfide‐linked homodimeric neurotrophic factor structurally related to GDNF, neurturin and persephin. These proteins belong to the cysteine knot superfamily of growth factors that assume stable dimeric protein structures. Artemin, GDNF, persephin and neurturin all signal through a multicomponent receptor system, composed of RET (receptor tyrosine kinase) and one of the four GFRα (α1‐α4) receptors. Artemin prefers the receptor GFRα3‐RET, but will use other receptors as an alternative. Artemin supports the survival of all peripheral ganglia, such as sympathetic, neural crest and placodally‐derived sensory neurons, and dopaminergic midbrain neurons. The functional human artemin ligand is a disulfide‐linked homodimer of two 12.0 kDa polypeptide monomers. Each monomer contains seven conserved cysteine residues, one of which is used for interchain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine‐knot configuration. Recombinant Murine Artemin is a disulfide‐linked homodimer formed by two identical 113 amino acid subunits with a calculated molecular weight of 24.2 kDa.
Product Specifications
Synonyms
ART, ARTN, Enovin, Neublastin
NCBI Gene ID
11876
UniProt
Q9Z0L2
Accession Number
NP_001271120.1
Accession Number mRNA
NM_001284191.1
Chromosomal Location
4; 4 D2.1
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
AGTRSSRART TDARGCRLRS QLVPVSALGL GHSSDELIRF RFCSGSCRRA RSQHDLSLAS LLGAGALRSP PGSRPISQPC CRPTRYEAVS FMDVNSTWRT VDHLSATACG CLG
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
≥ 97% by SDS-PAGE gel and HPLC analyses.
Bioactivity
Determined by the dose-dependent stimulation of the proliferation of SH-SY5Y cells. The expected ED50 for this effect is 0.1-0.5 µg/ml.
Length
113
Form
Lyophilized
Molecular Weight
24.2 kDa
Host or Source
E.coli
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