Recombinant EED Monoclonal Antibody
Product Specifications
Background
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119. Embryonic ectoderm development protein (EED) is a component of the PRC2 complex, which together with Ezh2 and SUZ12 is absolutely required for histone methyl-transferase activity. EED, SUZ12 and EZH2 are overexpressed in various types of cancer, including tumors of the colon, breast, prostate and ovary.
Abbreviation
EED
UniProt
O75530
Host
Rabbit
Reactivity
Human; Mouse
Immunogen
Peptide. This information is proprietary to PTMab.
Target
WAIT; EED; HEED; WAIT1; COGIS; embryonic ectoderm development; HWAIT1
Clonality
Monoclonal,Recombinant
Clone
A755
Conjugation
Unconjugated
Applications
WB
Field of Research
Epigenetics and Nuclear Signaling
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:1000
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Product MSDS
https://789.bio/eb/6xlRZtIG
Calculated Molecular Weight
50 kDa
Observed Molecular Weight
50-70 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
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