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Recombinant beta II Tubulin Monoclonal Antibody

Product Specifications

Background

Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. As an internal reference protein, the protein levels of β tubulin generally do not change. Therefore, it has been widely used as a reference for the consistency of sample loading in Western Bloting and also for immunostaining to observe the microtubule structure of cells.

Abbreviation

Beta II Tubulin

UniProt

Q9BVA1

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human beta II Tubulin fragment

Target

TUBB2B

Clonality

Monoclonal,Recombinant

Clone

A592

Conjugation

Unconjugated

Applications

WB; IHC; IP

Field of Research

Isotype; Loading Controls; Tags & Cell Markers; Signal Transduction

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IP 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

50 kDa

Observed Molecular Weight

55 kDa

Isotype

IgG, κ

Available Sizes

Frequently Asked Questions

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