Recombinant beta II Tubulin Monoclonal Antibody
Product Specifications
Background
Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. As an internal reference protein, the protein levels of β tubulin generally do not change. Therefore, it has been widely used as a reference for the consistency of sample loading in Western Bloting and also for immunostaining to observe the microtubule structure of cells.
Abbreviation
Beta II Tubulin
UniProt
Q9BVA1
Host
Rabbit
Reactivity
Human; Rat; Mouse
Immunogen
Recombinant human beta II Tubulin fragment
Target
TUBB2B
Clonality
Monoclonal,Recombinant
Clone
A592
Conjugation
Unconjugated
Applications
WB; IHC; IP
Field of Research
Isotype; Loading Controls; Tags & Cell Markers; Signal Transduction
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:200-1:1000; IP 1:50-1:100
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
50 kDa
Observed Molecular Weight
55 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
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