Recombinant SF3B3 Monoclonal Antibody
Product Specifications
Background
SF3B3 is one of four proteins for SF3B, which is involved in RNA splicing. Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occurred in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which composed of 4 proteins. SF3B3 is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA.
Abbreviation
SF3B3
UniProt
Q15393
Host
Rabbit
Reactivity
Human; Rat; Mouse
Immunogen
Recombinant human SF3B3 fragment
Target
SAP; SF3B; KIAA; Splicing factor 3B subunit; Spliceosome-associated protein; Spliceosome-associated protein 130; Splicing factor 3B subunit 3; SF3B3; KIAA0017; SAP130; Pre mRNA splicing factor SF3b 130 kDa subunit; Pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; SF3b130; SF3B3_HUMAN; Spliceosome associated protein 130; Splicing factor 3b subunit 3 130kD; STAF130
Clonality
Monoclonal,Recombinant
Clone
A553
Conjugation
Unconjugated
Applications
WB; IHC; IP
Field of Research
Epigenetics and Nuclear Signaling
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:500-1:1000; IHC 1:500-1:2000; IP 1:25
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Product MSDS
https://789.bio/eb/82HdhasP
Calculated Molecular Weight
135 kDa
Observed Molecular Weight
135 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
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