Recombinant Topoisomerase I Monoclonal Antibody
Product Specifications
Background
DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine.
Abbreviation
Topoisomerase I
UniProt
P11387
Host
Rabbit
Reactivity
Human; Rat; Mouse
Immunogen
Recombinant human Topoisomerase I fragment
Target
TOP; TOP1; TOPI
Clonality
Monoclonal,Recombinant
Clone
A475
Conjugation
Unconjugated
Applications
WB; IHC; IF; FCM; IP
Field of Research
Epigenetics and Nuclear Signaling; Cancer
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:100-1:500; IF 1:50; FCM 1:50-1:100; IP 1:25-1:50
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
91 kDa
Observed Molecular Weight
108 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
