Recombinant Smac/Diablo Monoclonal Antibody
Product Specifications
Background
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) . Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Abbreviation
Smac/Diablo
UniProt
Q9NR28
Host
Rabbit
Reactivity
Human; Rat; Mouse
Immunogen
Recombinant human Smac/Diablo fragment
Target
DFNA; DIABLO; DFNA64; SMAC;0610041G12Rik; DBLOH; DBOH; Diablo homolog; Diablo homolog (Drosophila) ; Diablo homolog mitochondrial; Diablo IAP binding mitochondrial protein; Diablo like protein; DIABLO S; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac protein; SMAC3
Clonality
Monoclonal,Recombinant
Clone
A361
Conjugation
Unconjugated
Applications
WB; IHC; IF; FCM; IP
Field of Research
Cell Biology; Cancer; Metabolism
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:50-1:100; IF 1:500-1:1000; FCM 1:50-1:100; IP 1:25-1:100
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
27 kDa
Observed Molecular Weight
21 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
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