Recombinant Phospho-Cyclin D1 (Thr286) Monoclonal Antibody

Product Specifications

Background

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Abbreviation

Phospho-Cyclin D1 (Thr286)

UniProt

P24385

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human Phospho-Cyclin D1 (Thr286) fragment

Target

Cyl; PRAD; U21B; CCND; B cell leukemia; G1/S specific cyclin D; Cyclin D; BCL; BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; B cell leukemia 1; BCL 1; Cd1; Cyl 1; G1/S specific cyclin D1; BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1

Clonality

Monoclonal,Recombinant

Clone

A202

Conjugation

Unconjugated

Applications

WB; IHC; FCM

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:100; FCM 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

34 kDa

Observed Molecular Weight

34 kDa

Isotype

IgG, κ

Available Sizes

Curated Selection

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