Rabbit anti Human Ig lambda light chain (free and bound)

The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of Human serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno- diffusion.

A pool of purified Bence Jones lambda proteins isolated from Human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.

Delipidated, heat inactivated lyophilized whole serum; No preservative added, as it may interfere with the antibody activity; It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Repeatedthawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the product