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Recombinant PCNA Monoclonal Antibody

Product Specifications

Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.

Abbreviation

PCNA

UniProt

P12004

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human PCNA Protein

Target

MGC; ATLD; etID36690.; HGCN; fa28e; fb36g; PCNA; ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03

Clonality

Monoclonal

Clone

3G1

Conjugation

Unconjugated

Applications

WB; IP

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Tags & Cell Markers; Cancer; Isotype; Loading Controls

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IP 1-5 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

35 kDa

Isotype

IgG

Frequently Asked Questions

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