Recombinant Phospho-NF-κB p65 (Ser536) Monoclonal Antibody
Product Specifications
Background
65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB.
Abbreviation
RELA
UniProt
Q04206
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65
Target
NFKB; Transcription factor p; Nuclear factor of kappa light polypeptide gene enhancer in B-cells; NF-kB p; NFKB3; p65; NF-kB p65; RELA; CMCU; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3; p65; transcription factor p65; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NF kappa B p65delta3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; OTTHUMP00000233473; OTTHUMP00000233474; OTTHUMP00000233475; OTTHUMP00000233476; OTTHUMP00000233900; p65 NF kappaB; p65 NFkB; TF65; V rel avian reticuloendotheliosis viral oncogene homolog A; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65) ) ; V rel reticuloendotheliosis viral oncogene homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian)
Clonality
Monoclonal
Clone
11G4
Conjugation
Unconjugated
Applications
WB
Field of Research
Cell Biology; Neuroscience; Microbiology; Signal Transduction; Epigenetics and Nuclear Signaling; Cancer; Cardiovascular; Metabolism
Purification
Protein A
Concentration
1 mg/mL
Dilution
WB 1:2000-1:20000
Buffer
10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5
Shipping Conditions
Ice bag
Storage Conditions
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
65 kDa
Observed Molecular Weight
70 kDa
Isotype
IgG
Available Sizes
Curated Selection
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