ATP Chemiluminescence Assay Kit (Double Reagent)
Product Specifications
Type
Fluorometric method
Field of Research
Oxidative Stress; Tricarboxylic Acid (TCA) Cycle; Mitochondria Function; Energy Metabolism
Assay Principle
Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content.
Assay Performance Time
40 min
Sample Type
Cell
Detection Range
0.002-1 μmol/L
Sensitivity
0.002 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
3.4-5.2%
Intra CV
1.7-2.6%
Detection Instrument
Chemiluminescence immunoassay analyzer; multifunctional microplate reader (with chemiluminescence)
Available Sizes
Frequently Asked Questions
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