Uncoated Human CCL15/MIP-5 (C-C motif chemokine 15/Macrophage inflammatory protein 5) ELISA Kit

HCC; LKN; Leukotactin; Chemokine CC; SCYA; C-C motif chemokine; Small-inducible cytokine A; Macrophage inflammatory protein; C-C motif chemokine 15; Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15; MIP5; NCC3; SCYA15; CCL15; HMRP-2B; MIP-1d; SCYL3

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL15/MIP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL15/MIP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL15/MIP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCL15/MIP-5. You can calculate the concentration of Human CCL15/MIP-5 in the samples by comparing the OD of the samples to the standard curve.