QuicKey Pro Human TG (Thyroglobulin) ELISA Kit
Product Specifications
Abbreviation
TG
UniProt
P01266
Cross Reactivity
This kit recognizes Human CXCL16 in samples. No significant cross-reactivity or interference between Human CXCL16 and analogues was observed.
Target
AITD3; TGN
Type
Sandwich-ELISA
Applications
ELISA
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.78-50 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.47 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum, plasma and other biological fluids.
Available Sizes
Curated Selection
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