SCD100 Recombinant Protein

The Semphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC "cooperation”. The CD100 precursor contains 862 amino acids, including a 21 a.a. signal sequence, a 713 a.a. extracellular domain, a 21 a.a transmembrane sequence, and a 107 a.a cytoplasmic region. The extracellular sequence contains several structural features, including a 479 a.a "sema” domain, a 79 a.a. Ig-like sequence, and a 52 a.a "Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids) . SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.

The lyophilized sCD100 recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted sCD100 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing sCD100 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.