TIM-1 Antibody

Product Specifications

Background

TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

NCBI Gene ID

171283

Swiss Prot

Q5QNS5

Accession Number

Q5QNS5.2

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

9927

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Relative Expression Validation (Figure 1) : Anti-TIM-1 antibodies (9927) specificity was further verified by TIM-1 relative expression. TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas. (https://www.proteinatlas.org/ENSG00000113249-HAVCR1/tissue) . Relative Expression Validation9927) TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas. (https://www.proteinatlas.org/ENSG00000113249-HAVCR1/tissue

Field of Research

Immunology, Neuroscience, Signal Transduction, Growth Factors

Purification

TIM-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1405 – Mouse Kidney lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Human: (63%) .

Buffer

TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TIM-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .