Tyrosine Hydroxylase Antibody

Product Specifications

Background

Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.

NCBI Gene ID

25085

Swiss Prot

P04177

Accession Number

P04177

Host

Sheep

Reactivity

Human, Mouse, Rat, Dog, Bovine

Immunogen

TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

Affinity Purified

Concentration

Batch dependent

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Calculated Molecular Weight

Applications Notes

Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB) . The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.

Symbol

NCBI Official Name

Tyrosine hydroxylase

NCBI Organism

Rattus norvegicus

Background Reference 01

Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.

Background Reference 02

Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies, " J. Neurochem. 60 (1993) 493-502.

Background Reference 03

Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla, " J. Neurochem. 56 (1991) 2139 - 2142.

Background Reference 04

Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody, " Anal. Biochem. 181 (1989) 259 - 266.