Tyrosine Hydroxylase Antibody
Product Specifications
Background
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.
NCBI Gene ID
25085
Swiss Prot
P04177
Accession Number
P04177
Host
Sheep
Reactivity
Human, Mouse, Rat, Dog, Bovine
Immunogen
TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience, Signal Transduction
Purification
Affinity Purified
Concentration
Batch dependent
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Calculated Molecular Weight
60
Applications Notes
Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB) . The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
Symbol
Th
NCBI Official Name
Tyrosine hydroxylase
NCBI Organism
Rattus norvegicus
Background Reference 01
Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.
Background Reference 02
Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies, " J. Neurochem. 60 (1993) 493-502.
Background Reference 03
Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla, " J. Neurochem. 56 (1991) 2139 - 2142.
Background Reference 04
Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody, " Anal. Biochem. 181 (1989) 259 - 266.
Tested Applications
WB, IF, IHC
Protein ID
136577
Physical Properties
Liquid
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