NMDA NR2A Antibody

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR2A subunit of the receptor has been associated synaptic plasticity.

NMDA NR2A Subunit antibody can be stored at -20˚ C. After reconstitution in 50 μ l PBS, the antibody should be aliquot and stored at -20˚ C and is stable at -20˚ C for at least 1 year. Ambient shipment is suitable for both international and domestic.

NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB) . Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 μ g/200 μ g lysate will quantitatively precipitate all NMDAR 2A for IP.

Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications, " Pharmacol. Ther., 97 (2003) 55 - 85.

Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C., and Browning, M.D., "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat, " J. Neurosci., 22 (2002) 3628 - 3637.

Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1, " Nature Neurosci., 5 (2002) 27 - 33.

Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L., "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion, " Mol. Brain Res., 40 (1996) 71 - 78.