NMDA NR1 Antibody

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR1 subunit of the receptor has been associated with synaptic plasticity. There are a number of different splice variants of the NR1. Differential splicing of three exons in the NR1 subunit generates up to eight NR1 splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2) . Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2') . Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

NMDA NR1, Splice Variant C2 antibody can be stored at -20˚ C. After reconstitution in 50 μ l PBS, the antibody should be aliquot and stored at -20˚ C and is stable at -20˚ C for at least 1 year. Ambient shipment is suitable for both international and domestic.

NMDA NR1, Splice Variant C2 antibody allows for strong and specific immunolabeling of the NR1 subunit containing the C2 splice variant insert. Does not recognize NR1 subunits that do not contain the C2 insert. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB) . Rabbit anti-NMDA NR1, Splice Variant C2 recognizes NR1 in rat and mouse brain. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1, " Nature Neurosci., 5 (2002) 27 - 33.

Luo, J.H., Wang, Y.H., Yasuda, R.P., Dunah, A.W. and Wolfe, B.B., "The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B), " Mol. Pharmacol. 51 (1997) 79 - 86.

Laurie, D.J. and Seeburg, P.H., "Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA, " J. Neurosci., 14 (1994) 3180 - 3194.