NMDA NR1 Antibody [R1JHL]

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR1 subunit of the receptor has been associated with synaptic plasticity.

NMDA NR1 Subunit antibody can be stored at -20˚ C. After reconstitution in 50 μ l water, the antibody should be aliquot and stored at -20˚ C. This product is stable at -20˚ C for at least 1 year and can be shipped ambient domestic or international.

NMDA NR1 Subunit antibody allows for strong and specific immunolabeling of the 120k NR1 protein in Western blots of rat brain hippocampal homogenate. Applications include Dot Blots (DB), Immunoprecipitation (IP) and Western Blots (WB) . Mouse anti-NMDA NR1 Subunit recognizes NR1 in human, mouse and rat brain lysates. When internally tested under ideal conditions the working dilutions were 1:300 for DB and WB, and 3 μ g per 200 μ g lysate (will quantitatively precipitate all the NMDA NR1 in 200 μ g of rat brain lysate) .

Luo, J.H., Wang, Y.H., Yasuda, R.P., Dunah, A.W. and Wolfe, B.B., "The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B), " Mol. Pharmacol. 51 (1997) 79 - 86.

Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1, " Nature Neurosci., 5 (2002) 27 - 33.