Glycine Receptor Antibody

Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult) .

Glycine Receptor antibody can be stored at -20˚ C. After reconstitution in 50 μ l PBS, the antibody should be aliquot and stored at -20˚ C. This product is stable at -20˚ C for at least 1 year.

Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB) . Rabbit anti-Glycine Receptor recognizes glycine receptor α1 and α2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Schofield, C.M., Jenkins, A. and Harrison, N.L., "A highly conserved aspartic acid residue in the signature disulfide loop of the a1 subunit is a determinant of gating in the glycine receptor, " J. Biol. Chem. 278 (2003) 34,079-34,083.

Meier, J., Vannier, C., Serge, A., Triller, A., and Choquet, D. "Fast and reversible trapping of surface glycine receptors by gephyrin, " Nat. Neurosci. 4 (2001) 253 - 260.

Beckstead, M.J., Weiner, J.L, Eger, E.I., Gong, D.H. and Mihic, S.J., "Glycine and gamma-aminobutyric acid (A) receptor function is enhanced by inhaled drugs of abuse, " Mol. Pharmacol. 57 (2000) 1199 - 1205.

Eggers, E.D., O'Brien, J.A., and Berger, A.J., "Developmental changes in the modulation of synaptic glycine receptors by ethanol, " J. Neurophysiol. 84 (2000) 2409 - 2416.