Dopamine b-Hydroxylase Antibody

Product Specifications

Background

Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. The expression of DBH is elevated during stress.

NCBI Gene ID

1621

Swiss Prot

P09172

Accession Number

P09172

Host

Sheep

Reactivity

Human, Rat, Sheep, Bovine

Immunogen

DBH polyclonal antibody was raised against a synthetic peptide from the N-terminal region of human DBH, conjugated to keyhole limpet hemocyanin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Affinity Purified

Concentration

Batch dependent

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Calculated Molecular Weight

Specificity

Dopamine b-Hydroxylase, NT antibody is specific immunolabeling of the ~75k DBH protein in Western blots of human adrenal medulla.

Applications Notes

Applications include Dot Blots (DB) and Western Blots (WB) . Sheep anti-DBH recognizes DBH in bovine, human, monkey and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Symbol

DBH

NCBI Official Name

Dopamine beta-hydroxylase (dopamine beta-monooxygenase)

NCBI Organism

Homo sapiens

Background Reference 01

Sabban, E.L., Kvetnansky, R. "Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events, " Trends Neurosci. 24 (2001) 91 - 98.

Background Reference 02

Nagatsu, I, Kobayashi, K, Fujii, T, Komori, K, Sekiguchi, K, Titani, K, Fujita, K, and Nagatsu, T. "Antibodies raised against different oligopeptide segments of human dopamine-β -hydroxylase, " Neuroscience Letters, 120 (1990) 141 - 145.