Tyrosine Hydroxylase (phospho Ser40) Antibody

Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.

TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH) .

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 μM for 60 minutes) . Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.

Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB) . Human, porcine, quail, mouse, and non-human primate have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.

Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina, " J. Chem. Neuroanat., 19 (2000) 105 - 116.

Haycock, J. W., Lew, J. Y., Garcia-Espana, A., Lee, K. Y., Harada, K., Meller, E., and Goldstein, M., "Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis, " J. Neurochem., 71 (1998) 1670-1675.

Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.