Synaptotagmin (phospho Ser309) Antibody

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.

Synaptotagmin (Ser309) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser309 of synaptotagmin.

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo, " Nature (London) 426 (2003) 559 - 563.

Wang, C.T., Lu, J.C., Bai, J.H., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion, " Nature (London) 424 (2003) 943 - 947.

Fernandez-Chacon, R., Konigstorfer, A., Gerber, S.H., Garcia, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Sudhof, T.C., "Synaptotagmin I functions as a calcium regulator of release probability, " Nature (London) 410 (2001) 41 - 49.

Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P., and Czernik, A.J., "Regulation of synaptotagmin I phosphorylation by multiple protein kinases, " J. Neurochem. 73 (1999) 921 - 932.