Synaptotagmin (phospho Thr202) Antibody

Product Specifications

Background

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.

NCBI Gene ID

25716

Swiss Prot

P21707

Accession Number

P21707

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synaptotagmin (Thr202) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr202 of synaptotagmin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Calculated Molecular Weight

60/62

Specificity

Synaptotagmin antibody is specific for Mr 60 - 62k synaptotagmin phosphorylated at Thr202.

Applications Notes

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Symbol

Syt1

NCBI Official Name

Synaptotagmin I

NCBI Organism

Rattus norvegicus

Background Reference 01

Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo, " Nature (London) 426 (2003) 559 - 563.

Background Reference 02

Wang, C.T., Lu, J.C., Bai, J.H., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion, " Nature (London) 424 (2003) 943 - 947.

Background Reference 03

Fernandez-Chacon, R., Konigstorfer, A., Gerber, S.H., Garcia, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Sudhof, T.C., "Synaptotagmin I functions as a calcium regulator of release probability, " Nature (London) 410 (2001) 41 - 49.

Background Reference 04

Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P., and Czernik, A.J., "Regulation of synaptotagmin I phosphorylation by multiple protein kinases, " J. Neurochem. 73 (1999) 921 - 932.