Progesterone Receptor (phospho Ser294) Antibody [608]

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms) . In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Progesterone receptor (Ser294) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer294of human progesterone receptor.

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB) . Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.

Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P., "Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody, " Mol. Endocrinol., 14 (2000) 52 - 65.

Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C., "Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion, " Endocrinology 144 (2003) 5650 -5657.