PLKK (phospho Ser482 / Ser486 / Ser490) Antibody

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

PLKK (Ser482/486/490) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus Phospho-Ser482, 486, 490 PLKK.

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Xenopus is the only species tested to date. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Van Vugt, M.A.T.M., van de Weerdt, B.C.M., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M.F., and Medema, R.H., "Polo-like Kinase-1 Is Required for Bipolar Spindle Formation but Is Dispensable for Anaphase Promoting Complex/Cdc20 Activation and Initiation of Cytokinesis, " J. Biol. Chem., 279 (2004) 36,841-36,854.

Erikson, E., Haystead, T.A.J., Qian, Y-W., and Maller, J.L., "A Feedback Loop in the Polo-like Kinase Activation Pathway, " J. Biol. Chem., 279 (2004) 32,219-32,224.

Liu, J., Lewellyn, A.L., Chen, L.G., and Maller, J.L., "The Polo Box Is Required for Multiple Functions of Plx1 in Mitosis, " J. Biol. Chem., 279 (2004) 21,367 - 21,373.

Kumagai, A., and Dunphy, W. G., "Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts, " Science 273 (1996) 1377-1380.