P53 (phospho Ser392) Antibody

P53 has a well established role in the blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition p53 has also been linked to affects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. We raised this polyclonal antibody against a peptide representing the sequence around Ser392 of p53.

P53 (Ser392) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53.

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB) . Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

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Jiang, Y.H., Armstrong, D., Albrecht, U., Atkins, C.M., Noebels, J.L., Eichele, G., Sweatt, J.D., and Beaudet, A.L., "Mutation of the angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation, " Neuron, 21 (1998) 799 - 811.

Pise-Masison, C.A., Radonovich, M., Sakaguchi, K., Appella, E., and Brady, J.N., "Phosphorylation of p53: a novel pathway for p53 inactivation in human T- cell lymphotropic virus type 1-transformed cells, " J. Virol., 72 (1998) 6348-6355.