DARPP 32 (phospho Thr75) Antibody

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.

DARPP-32 (Thr75) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse.

For long term storage -80˚ C is recommended, but shorter term storage at -20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚ C.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr75) recognizes human and mouse forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway, " Science, 302 (2003) 1412 - 1415.

Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.

Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens, " Nature Neurosci. 5 (2002) 641 - 648.