VEGF Antibody [4B2-8]

Product Specifications

NCBI Gene ID

7422

Swiss Prot

P15692

Accession Number

P15692

Host

Mouse

Reactivity

Human

Immunogen

The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.

Clonality

Monoclonal

Clone

4B2-8

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Signal Transduction, Growth Factors

Purification

This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.

Concentration

Batch dependent

Modification

None

Shipping Conditions

Room Temp

Storage Conditions

VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

Applications Notes

ELISA: In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.