APRIL Antibody [Sacha-1]

APRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL), and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5 μ g/mL Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line) . Cells were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μ g/mL of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.