BAFF Antibody [Buffy-2]

Product Specifications

NCBI Gene ID

10673

Swiss Prot

Q9Y275

Host

Rat

Reactivity

Human, Mouse

Immunogen

BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

Clonality

Monoclonal

Clone

Buffy-2

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Chemokines & Cytokines, Cancer

Purification

Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.

Concentration

Batch dependent

Buffer

PBS containing 0.02% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

BAFF antibody can be stored at 4˚ C, stable for one year.

Fragment

IgM

Applications Notes

BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..

NCBI Official Name

Tumor necrosis factor (ligand) superfamily, member 13b

NCBI Organism

Homo sapiens

Background Reference 01

Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002) .