BAFF Antibody [Buffy-1] (FITC)

Product Specifications

NCBI Gene ID

10673

Swiss Prot

Q9Y275

Host

Rat

Reactivity

Human

Immunogen

BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

Clonality

Monoclonal

Clone

Buffy-1

Conjugation

FITC

Type

Primary Antibodies

Field of Research

Chemokines & Cytokines, Cancer

Purification

Anti BAFF (monoclonal Buffy-1) antibody was purified to ≥95% in SDS-PAGE.

Concentration

Batch dependent

Buffer

PBS containing 0.02% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

BAFF antibody can be stored at 4˚ C, stable for one year.

Fragment

IgG2a

Applications Notes

BAFF antibody detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4 μ g/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.

NCBI Official Name

Tumor necrosis factor (ligand) superfamily, member 13b

NCBI Organism

Homo sapiens

Background Reference 01

BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999) .

Background Reference 02

BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000) .

Background Reference 03

A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) .