Perforin Antibody [CB5.4]

Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μ g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC) . Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993) .

Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller and J. Tschopp; J. Immunol. 153, 2470 (1994) .

Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004) .