SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8]

As SARS-CoV-2 Spike S2 protein is more conserved among coronaviruses than Spike S1 protein, antibodies against spike S2 protein may react with more Coronaviruses, serving as a pan-coronvirus antibody. The functional characteristics analysis revealed that P1B8 has excellent binding activity to SARS-CoV-2 variants (delta and omicron), but weaker binding activity to SARS-CoV-1. Biotinylated P1B8 can still bind spike trimer of SARS-CoV-2. In addition, P1B8 and another three spike S2 sdAbs (P1G5, P1A9, and P1A6) belong to different families based on their sequence alignment, and epitope mapping experiment indicated that they may bind to different epitopes with different affinity to the different strains in the binding ELISA. Our data showed that these spike S2 sdAbs bind spike trimer of both SARS-CoV-2 and SARS-CoV-1, suggesting that these spike S2 sdAbs have potentials as a novel and efficient tool suitable for pan-coronavirus studies as well as for identification of various coronaviruses and their variants from clinical samples.

SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293) . It contains amino acids Ser 686 - Pro 1213.

SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

SARS-CoV-2 (COVID-19) Spike S2 Antibody should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.